6533b821fe1ef96bd127c172

RESEARCH PRODUCT

Sodium Dodecylsulphate Electrophoresis

Gunter M. Rothe

subject

chemistry.chemical_classificationResidue (chemistry)chemistry.chemical_compoundElectrophoresisEnzymeMonomerChromatographychemistryMembrane proteinMolecular massSodiumProtein primary structurechemistry.chemical_element

description

Sodium dodecylsulphate (SDS) consists of an aliphatic chain of 12 C atoms to which at one end a sulphate residue is bound. It forms complexes with both the polar and non- polar amino acid residues of proteins irrespective of their sizes and shapes, leaving the primary structure uninfluenced. In electrophoresis SDS is used: a) to separate (enzyme) proteins into their monomeric constituents, b) to estimate the molecular mass of unfolded (and reduced) polypeptides, and c) to keep membrane proteins in a solubilized state.

yearjournalcountryeditionlanguage
1994-01-01
https://doi.org/10.1007/978-3-642-79069-0_4