6533b821fe1ef96bd127c2d1

RESEARCH PRODUCT

Morphometric Study of the Bone Marrow in Polycythemia Vera Following Interferon-Alpha Therapy

G. CampesiVito FrancoAda Maria FlorenaAragona F

subject

AdultMalePathologymedicine.medical_specialtyAlpha interferonCell CountPathology and Forensic MedicinePolycythemia veraBone MarrowFibrosishemic and lymphatic diseasesmedicineHumansMyelofibrosisPolycythemia VeraAgedAged 80 and overbusiness.industryInterferon-alphaCell BiologyMiddle Agedmedicine.diseaseBone marrow cellularitymedicine.anatomical_structurePrimary MyelofibrosisMarrow fibrosisFemaleBone marrowbusinessMegakaryocytesImmunostaining

description

Bone marrow cellularity and extent of fibrotic change were determined in nineteen patients with polycythemia vera, treated with interferon-alpha (IFN) for 1 year. The cellularity was evaluated with an interactive semiautomatic method using Leitz TAS plus microscope: in particular, number and size of megakaryocytes were evaluated after immunostaining with Y2/51 (CD 61); reticulin content was studied by light microscope with a semiquantitative method. Before IFN therapy mean cellularity was 80.5% (+/- 13.7). After 6 and 12 months mean cellularity was 75.4% and 68.4% respectively. Six months after cessation of IFN therapy the cellularity was 69.1%. A decrease of the number, density and morphometrical parameters of megakaryocytes was also remarked. Reticulin fibrosis was mild in 13 cases and moderate in 6 cases before IFN therapy. Reticulin content was unmodified during therapy in all cases but two, in which fibrosis changed from mild to moderate. In conclusion IFN therapy is to be considered a good method in polycythemia vera for the control of proliferative activity of bone marrow but with IFN therapy one cannot determine regression of marrow fibrosis.

yearjournalcountryeditionlanguage
1993-02-01Pathology - Research and Practice
https://doi.org/10.1016/s0344-0338(11)80116-0