6533b821fe1ef96bd127c30d
RESEARCH PRODUCT
TAF-ChIP: an ultra-low input approach for genome-wide chromatin immunoprecipitation assay
Steffen AlbrechtPiyush MoreWaldemaar KaiserMarion SiliesJunaid AkhtarChristian BergerJean-fred FontaineLeszek WojnowskiFederico MariniApurva KulkarniMiguel A. Andrade-navarrosubject
Health Toxicology and MutagenesisPlant ScienceComputational biologySignal-To-Noise RatioBiochemistry Genetics and Molecular Biology (miscellaneous)GenomeDNA sequencingEpigenesis GeneticHistones03 medical and health sciences0302 clinical medicineTranscriptional regulationMethodsAnimalsHumansEpigenetics030304 developmental biologyWhole genome sequencing0303 health sciencesEcologybiologyWhole Genome SequencingChemistryHigh-Throughput Nucleotide SequencingChip11Histonebiology.proteinChromatin Immunoprecipitation SequencingDrosophilaK562 CellsChromatin immunoprecipitation030217 neurology & neurosurgerySoftwaredescription
The authors present a novel method for obtaining chromatin profiles from low cell numbers without prior nuclei isolation. The method is successfully implemented in generating epigenetic profile from 100 cells with high signal-to-noise ratio.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2019-07-01 | Life Science Alliance |