A method for measuring mitochondrial mass and activity

6533b821fe1ef96bd127c322

RESEARCH PRODUCT

A method for measuring mitochondrial mass and activity

Anna Maria Rinaldi Giovanni Morici Maria Agnello

subject

Confocal Clinical Biochemistry Biomedical Engineering sea urchin.Bioengineering Mitochondrion mitochondrial activity Paracentrotus lividus MitoTracker Green Human fertilization In vivo biology.animal Settore BIO/06 - Anatomia Comparata E Citologia Sea urchin mitochondrial ma biology Brief Report Embryo Cell Biology Anatomy biology.organism_classification Fluorescence Cell biology MitoTracker Orange CLSM; mitochondrial mass; mitochondrial activity; MitoTracker Green; MitoTracker Orange; sea urchin.CLSM Biotechnology

description

Mitochondria, responsible for the energy-generating process essential for the cell metabolism, differ for the number, localization and activity in animal cells and tissues in relation to the energetic needs. Using fluorescent probes specific for mitochondria, Mitotracker Green (MTG) and Orange (MTO), and Confocal Laser-Scanning Microscope (CLSM), we elaborated a method to measure in vivo the mitochondrial mass and activity, in sea urchin Paracentrotus lividus eggs and embryos. The analysis of captured images, revealed a variation of mitochondrial distribution and an increase of activity after fertilization.

year	journal	country	edition	language
2007-11-23	Cytotechnology

https://doi.org/10.1007/s10616-008-9143-2

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