6533b821fe1ef96bd127c497

RESEARCH PRODUCT

Gamma enolase expression as early marker of neuronal differentiation of murine neuroblastoma cells N-115

Serafina SciarrinoAgata GiallongoL. D’amelioV. MatrangaMelchiorre Cervello

subject

Messenger RNAPeriod (gene)Clinical BiochemistryNeuronal differentiationBiomedical EngineeringBioengineeringCell BiologyMouse NeuroblastomaBiologyGamma-EnolaseMolecular biologyMurine neuroblastomaCell cultureNorthern blotBiotechnology

description

In this study we determined the levels of gamma enolase mRNA in mouse neuroblastoma cell line N-115 at early period of induction of differentiation by serum withdrawal. The expression of gamma enolase was examined by Northern blot analysis of total RNA extracted from cells induced for different lengths of time. We found a 3-fold increase in the level of gamma enolase mRNA after 24 hours of induction of differentiation and higher levels were detected in cells induced for longer time, reaching a 10-fold increase after four days.

yearjournalcountryeditionlanguage
2012-02-24Cytotechnology
https://doi.org/10.1007/bf00746095