6533b822fe1ef96bd127cb02

RESEARCH PRODUCT

Kupffer Cell and Monocyte-Derived Macrophage Identification by Immunofluorescence on Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse Liver Sections

Chloé BuchAdrien GuillotTony Jourdan

subject

0301 basic medicinePathologymedicine.medical_specialtymedicine.diagnostic_testFormalin fixed paraffin embeddedMonocyte derivedChemistryMonocyteKupffer cellImmunofluorescencelaw.invention03 medical and health sciences030104 developmental biology0302 clinical medicinemedicine.anatomical_structureConfocal microscopylaw030220 oncology & carcinogenesismedicineMacrophageImmunohistochemistry

description

Kupffer cells are the liver-resident macrophages and represent the first line of defense between the pathogens circulating from the intestines through the portal vein and systemic circulation. Recent works have highlighted the complex heterogeneity of macrophage functions and origins, thus raising awareness on the need for a better characterization of macrophage populations. The immunohistochemistry method here described, allows for a rapid distinction between Kupffer cells and monocyte-derived macrophages present on formalin-fixed, paraffin-embedded mouse liver samples. This protocol has been optimized for its reproducibility, reliability, and simplicity.

yearjournalcountryeditionlanguage
2020-01-01
https://doi.org/10.1007/978-1-0716-0704-6_6