6533b822fe1ef96bd127cc7d

RESEARCH PRODUCT

Regulation of the HTRA2 Protease Activity by an Inhibitory Antibody-Derived Peptide Ligand and the Influence on HTRA2-Specific Protein Interaction Networks in Retinal Tissues.

Kristian Nzogang FomoNatarajan PerumalFranz H. GrusNorbert PfeifferCarsten Schmelter

subject

retinaImmunoprecipitationQH301-705.5medicine.medical_treatmentMedicine (miscellaneous)PeptideAggrephagyNeuroprotectioninteraction partnersGeneral Biochemistry Genetics and Molecular BiologyArticlemedicineBiology (General)mass spectrometrychemistry.chemical_classificationProteaseHTRA2Neurodegenerationco-immunoprecipitationmedicine.diseaseProtease inhibitor (biology)Cell biologychemistryneuroprotectionTarget proteinmedicine.drug

description

The mitochondrial serine protease HTRA2 has many versatile biological functions ranging from being an important regulator of apoptosis to being an essential component for neuronal cell survival and mitochondrial homeostasis. Loss of HTRA2 protease function is known to cause neurodegeneration, whereas overactivation of its proteolytic function is associated with cell death and inflammation. In accordance with this, our group verified in a recent study that the synthetic peptide ASGYTFTNYGLSWVR, encoding the hypervariable sequence part of an antibody, showed a high affinity for the target protein HTRA2 and triggered neuroprotection in an in vitro organ culture model for glaucoma. To unravel this neuroprotective mechanism, the present study showed for the first time that the synthetic CDR1 peptide significantly (p &lt

yearjournalcountryeditionlanguage
2021-08-13Biomedicines
10.3390/biomedicines9081013https://pubmed.ncbi.nlm.nih.gov/34440217