6533b822fe1ef96bd127cdec
RESEARCH PRODUCT
Optimization of the Hydrolysis of Safflower Oil for the Production of Linoleic Acid, Used as Flavor Precursor
Marya AzizFlorence HussonSelim Kermashasubject
0106 biological sciencesArticle SubjectLinoleic acidlcsh:TX341-64101 natural sciencesHydrolysischemistry.chemical_compound010608 biotechnology[SDV.IDA]Life Sciences [q-bio]/Food engineeringFlavorchemistry.chemical_classificationChromatographylcsh:TP368-456010405 organic chemistryChemistrySubstrate (chemistry)0104 chemical sciencesCandida rugosalcsh:Food processing and manufactureEnzymeBiochemistryBiocatalysisLiberationlcsh:Nutrition. Foods and food supply[SDV.AEN]Life Sciences [q-bio]/Food and NutritionResearch ArticleFood Sciencedescription
Commercial lipases, from porcine pancreas (PPL),Candida rugosa(CRL), andThermomyces lanuginosus(Lipozyme TL IM), were investigated in terms of their efficiency for the hydrolysis of safflower oil (SO) for the liberation of free linoleic acid (LA), used as a flavor precursor. Although PPL, under the optimized conditions, showed a high degree of hydrolysis (91.6%), its low tolerance towards higher substrate concentrations could limit its use for SO hydrolysis. In comparison to the other investigated lipases, Lipozyme TL IM required higher amount of enzyme and an additional 3 h of reaction time to achieve its maximum degree of SO hydrolysis (90.2%). On the basis of the experimental findings, CRL was selected as the most appropriate biocatalyst, with 84.1% degree of hydrolysis. The chromatographic analyses showed that the CRL-hydrolyzed SO is composed mainly of free LA.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2015-06-01 | International Journal of Food Science |