6533b822fe1ef96bd127d32a

RESEARCH PRODUCT

LC-ESI/HRMS analysis of glucosinolates, oxylipins and phenols in Italian rocket salad (Diplotaxis erucoides subsp. erucoides (L.) DC.) and evaluation of its healthy potential

Loizzo Monica RNapolitano AssuntaBruno MaurizioGeraci AnnaSchicchi RosarioLeporini MariarosariaTundis RosaPiacente Sonia

subject

Glucosinolatesantioxidant activityantioxidant activity; Diplotaxis erucoides; hypoglycemic and hypolipidemic effect; LC-ESI/HRMS analysis; Antioxidants; Brassicaceae; Chromatography Liquid; Enzyme Inhibitors; Flavonoids; Glucosinolates; Glycoside Hydrolase Inhibitors; Humans; Mass Spectrometry; Oxylipins; Plant Extracts; Salads; Sicily; alpha-Amylases; alpha-GlucosidasesAntioxidantsMass SpectrometrySettore BIO/01 - Botanica GeneraleLC-ESI/HRMS analysisHumansGlycoside Hydrolase InhibitorsOxylipinsEnzyme InhibitorsSicilyFlavonoidsChromatographyLiquidPlant ExtractsSettore BIO/02 - Botanica Sistematicaalpha-GlucosidasesSettore CHIM/06 - Chimica Organicahypoglycemic and hypolipidemic effectBrassicaceaeSettore BIO/03 - Botanica Ambientale E ApplicataDiplotaxis erucoidesSaladsalpha-AmylasesChromatography Liquid

description

BACKGROUND: This study investigated the chemical profile and biological activity of Diplotaxis erucoides subsp. erucoides (L.) DC. (Brassicaceae) collected in Sicily (Italy). RESULTS: Liquid chromatography coupled with electrospray ionization and high-resolution mass spectrometry (LC-ESI/HRMS) analysis of the ethanol extract revealed the presence of 42 compounds – glucosinolates, hydroxycinnamic acids, flavonoids, and oxylipins. The extract was tested for its antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic) acid (ABTS), ferric reducing ability power (FRAP), and β-carotene bleaching tests. Promising protection from lipid peroxidation was observed after 30 min of incubation in a β-carotene bleaching test (IC50 of 3.32 μg mL−1). The inhibition of carbohydrates-hydrolyzing enzymes resulted in IC50 values of 85.18 and 92.36 μg mL−1 for α-amylase and α-glucosidase, respectively. Significant inhibition against lipase enzyme was observed (IC50 of 61.27 μg mL−1). CONCLUSION: Diplotaxis erucoides can be considered a potential source of antioxidant, hypoglycemic, and hypolipidemic bioactives. © 2021 Society of Chemical Industry.

yearjournalcountryeditionlanguage
2021-03-23
10.1002/jsfa.11239http://hdl.handle.net/10447/517577