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RESEARCH PRODUCT

Concentration-Dependent Protection by Ethanol Extract ofPropolis against &#947

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[EN] Radioprotection with natural products may be relevant to the mitigation of ionizing radiation-induced damage in mammalian systems; in this sense, propolis extracts have shown effects such as antioxidant, antitumoral, anti-inflammatory, and immunostimulant. We report for the first time a cytogenetic study to evaluate the radioprotective effect, in vitro, of propolis against radiation-induced chromosomal damage. Lymphocytes were cultured with increasing concentrations of ethanol extract of propolis (EEP), including 20, 40, 120, 250, 500, 750, 1000, and 2000 ¿g mL-1 and then exposed to 2 Gy ¿-rays. A significant and concentration-dependent decrease is observed in the frequency of chromosome aberrations in samples treated with EEP. The protection against the formation of dicentrics was concentration-dependent, with a maximum protection at 120 ¿g mL-1 of EEP. The observed frequency of dicentrics is described as negative exponential function, indicating that the maximum protectible fraction of dicentrics is approximately 44. Free radical scavenging and antioxidant activities are the mechanisms that these substances use to protect cells from ionizing radiation. Copyright © 2011 A. Montoro et al. This research was supported in part by the Company Dieteticos Intersa S.A., U.P.V., and Hospital Universitario La Fe of Valencia and Consejo de Seguridad Nuclear (2696/SRO). The authors express their appreciation to Dr. Pepe Perez (Hospital Universitario La Fe) and Dr. Alberto Yuste (U.P.V.).