A rapid method for the screening of plasmids in transformed yeast strains

6533b822fe1ef96bd127d5ba

RESEARCH PRODUCT

A rapid method for the screening of plasmids in transformed yeast strains

subject

Genetics Saccharomyces cerevisiae General Medicine Biology medicine.disease_cause biology.organism_classification Applied Microbiology and Biotechnology Microbiology Enterobacteriaceae Yeast Transformation (genetics)Plasmid Restriction map medicine Alkaline lysis Escherichia coli

description

A method for the rapid screening of plasmids in yeast cells has been developed. The method is an adaptation of the currently used alkaline lysis methods forEscherichia coli plasmids. Following the conditions described, several dozen ofSaccharomyces cerevisiae-transformed clones can be analyzed for their plasmid content in less than 2 h. The plasmids obtained by this procedure are suitable for restriction analysis or forE. coli andS. cerevisiae transformation.

year	journal	country	edition	language
1988-01-01	Current Microbiology

https://doi.org/10.1007/bf01568813

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