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RESEARCH PRODUCT
Insulin synthesis in chick embryo retinas during development.
Maria CarabillòMarianna LauricellaRenza VentoMichela GiulianoGiuseppe CalvarusoGiovanni Tesorieresubject
medicine.medical_specialtyInsulinmedicine.medical_treatmentEmbryogenesisEmbryoGeneral MedicineChick EmbryoBiologyBiochemistryIn vitroRetinaCellular and Molecular NeuroscienceEndocrinologyInsulin synthesisInternal medicineCulture TechniquesmedicineLiberationAnimalsInsulinsense organsLeucineIncubationExplant culturedescription
Retinas of chick embryos contain insulin (1) and further, are capable of synthesizing it, as demonstrated by incubating retinas at different ages (7th–18th day) with [3H]leucine. The synthesized radioactive insulin was isolated and assayed by means of a HPLC procedure. The synthesis of insulin was found to be highest in the youngest retinas studied (day 7), afterwards it declined with age except for an increment found at 14–15 day. Explants of chick embryo retinas, cultured in vitro, rapidly degraded insulin. Nevertheless, the content of immunoreactive insulin in retinal explants diminished slowly with the age of culture, so that, after 8 days of incubation, it was about 60% of the content found in the retinas at the beginning of incubation. This was proof that cultured explants are capable of efficiently synthesizing insulin. The synthesized [3H]insulin was released from explants into the medium. This was evident also after 6–8 days in culture.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1994-07-01 | Neurochemical research |