6533b822fe1ef96bd127d814
RESEARCH PRODUCT
α7 Nicotinic acetylcholine receptors and modulation of gabaergic synaptic transmission in the hippocampus
Manickavasagom AlkondonEdson X. AlbuquerqueEdson X. AlbuquerqueMaria F.m BragaMaria F.m BragaAlfred MaelickeEdna F. R. Pereirasubject
Agonistmedicine.medical_specialtyalpha7 Nicotinic Acetylcholine ReceptorInterneuronmedicine.drug_classAction PotentialsIn Vitro TechniquesReceptors NicotinicBiologyHippocampusSynaptic TransmissionCholinechemistry.chemical_compoundGanglion type nicotinic receptorInterneuronsInternal medicinemedicineAnimalsNeurotransmittergamma-Aminobutyric AcidPharmacologyMethyllycaconitineDose-Response Relationship DrugRatsElectrophysiologyEndocrinologymedicine.anatomical_structureNicotinic agonistchemistryBiophysicsAlpha-4 beta-2 nicotinic receptorAcetylcholinemedicine.drugdescription
The present report provides new findings regarding modulation of gamma-aminobutyric acid (GABA) transmission by alpha7 nicotinic receptor activity in CA1 interneurons of rat hippocampal slices. Recordings were obtained from tight-seal cell-attached patches of the CA1 interneurons, and agonists were delivered to the neurons via a modified U-tube. Application for 6 s of the alpha7 nicotinic receptor-selective agonist choline (or =1 mM) to all CA1 interneurons tested triggered action potentials that were detected as fast current transients. The activity triggered by choline terminated well before the end of the agonist pulse, was blocked by the alpha7 nicotinic receptor antagonist methyllycaconitine (50 nM) and was concentration dependent; the higher the concentration of choline the higher the frequency of events and the shorter the delay for detection of the first event. In 40% of the neurons tested, choline-triggered action potentials decreased in amplitude progressively until no more events could be detected despite the presence of the agonist. Primarily, this finding could be explained by Na(+)-channel inactivation associated with membrane depolarization induced by alpha7 nicotinic receptor activation. In 60% of the neurons, the amplitude of choline-induced action potentials was sustained at the intial level, but again the activity did not last as long as the agonist pulse, in this case apparently because of agonist-induced receptor desensitization. These results altogether demonstrate that agonists interacting with alpha7 nicotinic receptors, including the natural transmitter acetylcholine and its metabolite choline, influence GABAergic transmission, not only by activating these receptors, but also by controlling the rate of Na(+)-channel inactivation and/or by inducing receptor desensitization.
year | journal | country | edition | language |
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2000-03-01 | European Journal of Pharmacology |