6533b822fe1ef96bd127d8d2
RESEARCH PRODUCT
Development of a Sensitive Detection Method for Alphaviruses and Its Use as a Virus Neutralization Assay
Barbara S. SchnierleMario PerkovicChristin Schmidtsubject
0301 basic medicineviruses030106 microbiologyAlphavirusCross ReactionsBiologyAntibodies Viralmedicine.disease_causeSensitivity and SpecificityMicrobiologyArticleVirusCell LineMice03 medical and health sciencesTranscription (biology)VirologymedicineRoss River virusAnimalsHumansSerologic TestsLuciferaseChikungunyaLuciferasesSubgenomic mRNAMice Inbred BALB Cchikungunya virusAlphavirus InfectionsStructural geneRNAsubgenomic promoterTransfectionAntibodies NeutralizingVirologyMayaro virusQR1-502030104 developmental biologyInfectious DiseasesRoss River virus ; Mayaro virus ; Virusinfektion ; chikungunya virus ; subgenomic promoterImmunoglobulin MImmunoglobulin GRNA Viraldescription
Alphaviruses have a single-stranded, positive-sense RNA genome that contains two open reading frames encoding either the non-structural or the structural genes. Upon infection, the genomic RNA is translated into the non-structural proteins (nsPs). NsPs are required for viral RNA replication and transcription driven from the subgenomic promoter (sgP). Transfection of an RNA encoding the luciferase gene under the control of the sgP into cells enabled the detection of replication-competent chikungunya virus (CHIKV) or Mayaro virus (MAYV) with high sensitivity as a function of the induced luciferase activity. This assay principle was additionally used to analyze virus-neutralizing antibodies in sera and might be an alternative to standard virus neutralization assays based on virus titration or the use of genetically modified tagged viruses.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2021-06-22 | Viruses |