6533b823fe1ef96bd127dfc5

RESEARCH PRODUCT

FT-IR spectroscopic analysis for studying Clostridium cell response to conversion of enzymatically hydrolyzed hay

Linda MezuleMara GrubeMara GrubeTalis JuhnaKristina TihomirovaAlina NescereckaMarita GavareMarita Gavare

subject

Growth mediumClostridium acetobutylicumbiologyButanolOrganic Chemistryfood and beveragesAcetone–butanol–ethanol fermentationbiology.organism_classificationAnalytical ChemistryInorganic Chemistrychemistry.chemical_compoundClostridiumClostridium beijerinckiichemistryBiochemistryHayFood scienceCelluloseSpectroscopy

description

Abstract Grass hay is one of assailable cellulose containing non-food agricultural wastes that can be used as a carbohydrate source by microorganisms producing biofuels. In this study three Clostridium strains Clostridium acetobutylicum, Clostridium beijerinckii and Clostridium tetanomorphum, capable of producing acetone, butanol and ethanol (ABE) were adapted to convert enzymatically hydrolyzed hay used as a growth media additive. The results of growth curves, substrate degradation kinetics and FT-IR analyses of bacterial biomass macromolecular composition showed diverse strain-specific cell response to the growth medium composition.

https://doi.org/10.1016/j.molstruc.2012.12.016