showing 5 related works from this author
Hydrogen-producingEscherichia colistrains overexpressing lactose permease: FT-IR analysis of the lactose-induced stress
2014
The lactose permease gene (lacY) was overexpressed in the septuple knockout mutant of Escherichia coli, previously engineered for hydrogen production from glucose. It was expected that raising the lactose transporter activity would elevate the intracellular lactose concentration, inactivate the lactose repressor, induce the lactose operon, and as a result stimulate overall lactose consumption and conversion. However, overexpression of the lactose transporter caused a considerable growth delay in the recombinant strain on lactose, resembling to some extent the "lactose killing" phenomenon. Therefore, the recombinant strain was subjected to selection on lactose-containing media. Selection on …
FT-IR spectroscopic analysis for studying Clostridium cell response to conversion of enzymatically hydrolyzed hay
2013
Abstract Grass hay is one of assailable cellulose containing non-food agricultural wastes that can be used as a carbohydrate source by microorganisms producing biofuels. In this study three Clostridium strains Clostridium acetobutylicum, Clostridium beijerinckii and Clostridium tetanomorphum, capable of producing acetone, butanol and ethanol (ABE) were adapted to convert enzymatically hydrolyzed hay used as a growth media additive. The results of growth curves, substrate degradation kinetics and FT-IR analyses of bacterial biomass macromolecular composition showed diverse strain-specific cell response to the growth medium composition.
Minimum information about a biofilm experiment (MIABiE): standards for reporting experiments and data on sessile microbial communities living at inte…
2014
The minimum information about a biofilm experiment (MIABiE) initiative has arisen from the need to find an adequate and scientifically sound way to control the quality of the documentation accompanying the public deposition of biofilm-related data, particularly those obtained using high-throughput devices and techniques. Thereby, the MIABiE consortium has initiated the identification and organization of a set of modules containing the minimum information that needs to be reported to guarantee the interpretability and independent verification of experimental results and their integration with knowledge coming from other fields. MIABiE does not intend to propose specific standards on how biof…
Rapid Catalytic Water Disinfection from Earth Abundant Ca 2 Fe 2 O 5 Brownmillerite
2021
Water disinfection is a crucial challenge for humanity. Approaches that are effective, cheap, environmentally friendly, and do not promote gene exchange between bacteria are urgently required. Strongly oxidizing radicals are highly promising to achieve this as they lead to bacterial activation at high efficiencies. However, sources to consistently generate these radicals are limited to high energy UV/H2O2 treatments requiring a large energy input. Here the use of abundant, cheap, brownmillerite (Ca2Fe2O5) is demonstrated as an efficient radical generation material under dark conditions, showing a seven order of magnitude decrease in bacterial concentration over 10 min. This decrease is attr…
Sorption of humic substances on aquifer material at artificial recharge of groundwater
2003
Experiments in batch equilibrium system were carried out to evaluate the importance of physical and chemical factors determining the sorption efficiency of humic substances (HS) on aquifer material, which has been used for artificial recharge of groundwater (ARG) in drinking water production. Results showed that an increase of the amount of clay in the aquifer material and a decrease of pH in water increased the sorption efficiency. The sorption of higher molecular weight, more hydrophobic and aromatic HS (Aldrich and forest soil humic acids) were greater than the sorption of acidic HS (river fulvic acids), either on the aquifer material or to its representative sorbing phases, clay and org…