6533b823fe1ef96bd127e164
RESEARCH PRODUCT
Surface proteomic analysis of differentiated versus stem-like osteosarcoma human cells.
Marica GemeiClaudia CorboRenza VentoRosa Di NotoLuigi Del VecchioFrancesca D'alessiosubject
ProteomicsSurface phenotypeProteomeBiologyProteomicsStem cell markerBiochemistryCancer stem cellSettore BIO/10 - BiochimicamedicineHumansCancer stem cell Cell biology Osteosarcoma Surface proteomeProtein Interaction MapsMolecular BiologyOsteosarcomaKinaseMembrane ProteinsCell Differentiationmedicine.diseaseCell biologyMembrane proteinCell cultureNeoplastic Stem CellsOsteosarcomacancer stem cells proteomics osteosarcomadescription
Cancer stem cell characterization represents a breakthrough in cancer research. Despite evidence showing the existence and the role of cancer stem cells in osteosarcoma (OS) onset and progression, little is known about their specific surface phenotype. To address this issue, we carried out a cytometric analysis with an antibody-array comprising 245 membrane proteins comparing the stem and differentiated OS cells. As experimental model, we chose the stem-like cell line 3aminobenzamide-OS and its parental, differentiated, cell line MG63. We identified 50 differentially expressed, 23 homogeneously expressed, and 172 not expressed proteins in the two cell line models, thus defining a surface protein signature specific for each of them. Furthermore, we selected ERK1/2 (p44/42 mitogen-activated protein kinases) as a potential pathway correlated with processes that characterize tumorigenic potential and stemness of 3aminobenzamide-OS cells.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2013-01-01 |