6533b823fe1ef96bd127e176
RESEARCH PRODUCT
Diversity of extracellular vesicles from different developmental stages of Fasciola hepatica
Dolores BernalFernando CantalapiedraLidia JaraMaría TrelisChristian M. Sánchez-lópezAntonio Marcillasubject
0301 basic medicineFuture studies030231 tropical medicineNanoparticle tracking analysisExtracellular vesicles03 medical and health sciencesExtracellular Vesicles0302 clinical medicineHepaticaSize exclusion chromatographyparasitic diseasesFasciola hepaticaParasite hostingAnimalsSecretionLife Cycle StagesbiologyVesicleFasciola hepaticaExtracellular vesiclesbiology.organism_classificationCell biology030104 developmental biologyInfectious DiseasesFasciola hepatica juvenilesParasitologydescription
The secretion of extracellular vesicles (EVs) in Fasciola hepatica adult worms was described by our group in 2012. Since then, EVs have been found in other helminths, thus providing a new paradigm for the complete understanding of host-parasite communication. However, information was lacking regarding the possible existence and role of EVs from other developmental stages of the parasite. In this study, we confirm the secretion of EVs by F. hepatica eggs and juvenile forms. EVs were isolated by size exclusion chromatography and characterised by nanoparticle tracking analysis and electron microscopy. We observed a large diversity in the morphologies of these EVs, suggesting specific functions for different subpopulations, as has been proposed in other model systems. The identification of these populations of morphologically diverse EVs will facilitate future studies aimed at biochemically characterising the different classes of these vesicles as a first step in deciphering their role in host-parasite communication. (C) 2020 Australian Society for Parasitology. Published by Elsevier Ltd. All rights reserved.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2020-01-09 |