6533b823fe1ef96bd127e26f
RESEARCH PRODUCT
Minimal peripheral blood cells carrying clonal markers of b cell disorders: Evidence for monoclonality of circulating lymphocytes in patients with multiple myeloma
Albrecht LindemannWolfgang OsterFriedhelm HerrmannRoland Mertelsmannsubject
Immunoglobulin genemedicine.drug_classBiologyMonoclonal antibodyPeripheral blood mononuclear cellmedicineHumansCloning MolecularB cellMultiple myelomaB-LymphocytesAntibodies MonoclonalDNACell Biologymedicine.diseaseMolecular biologyClone CellsBlotting SouthernPhenotypemedicine.anatomical_structureImmunologyMonoclonalLeukocytes Mononuclearbiology.proteinBone marrowAntibodyMultiple MyelomaBiomarkersdescription
Peripheral blood lymphocytes (PBL) of 20 patients with multiple myeloma (MM) were assayed for clonality by Southern blot and cell surface marker analysis. Eight samples showed monoclonal origin of circulating lymphocytes by demonstrating rearrangements of the heavy chain immunoglobulin gene (IgH). In selected experiments, comparison of IgH rearrangements of bone marrow plasma cells and peripheral blood-derived mononuclear cells, highly enriched for B lymphocytes, proved to be identical. However, monoclonal circulating cells could not be detected in samples with rearranged IgH genes by surface marker phenotyping using one-color immunofluorescence analysis and a panel of monoclonal and polyclonal antibodies to various B lineage-associated antigens. These results indicate that in a substantial proportion of MM, monoclonal growth involves circulating B lymphocytes and underscores the clinical usefulness of Southern analysis of IgH gene rearrangements for monitoring this disease.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1989-03-01 | The International Journal of Cell Cloning |