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RESEARCH PRODUCT
In vitro effects of aminobisphosphonates on Vgamma9Vdelta2 T cell activation and differentiation.
G. Di FedeAlfredo SalernoRini GbG. Di LorenzoViviana FerlazzoFrancesco DieliSerena MeravigliaC. SferrazzaM. D'asaroNadia Caccamosubject
Cell SurvivalT cellT-LymphocytesImmunologyLymphocyte ActivationPeripheral blood mononuclear cellMonocytesFlow cytometry03 medical and health sciencesInterferon-gamma0302 clinical medicinemedicineImmunology and AllergyCytotoxic T cellHumansCytotoxicityImage CytometryPharmacologymedicine.diagnostic_testBone Density Conservation AgentsDiphosphonatesChemistryEffectorCell DifferentiationFlow CytometryPhenotypeIn vitromedicine.anatomical_structure030220 oncology & carcinogenesisCancer research030215 immunologydescription
In this study we have evaluated the in vitro effects of four different aminobisphosphonates, alendronate, risedronate, neridronate and zoledronate, on Vγ9Vδ2 T cell activation and differentiation. All tested aminobisphosphonates induce an IL-2-dependent activation and expansion of Vγ9Vδ2 T lymphocytes in primary PBMC cultures of healthy donors. Most notably, they also determine a different distribution of Vγ9Vδ2 T cell subsets, with decrease of Tnaive and TCM cells and increase of TEM and TEMRA Vγ9Vδ2 cells, indicating that in vitro treatment with aminobisphosphonates induces Vγ9Vδ2 T lymphocytes to differentiate towards an effector/cytotoxic phenotype. Accordingly, Vγ9Vδ2 T lymphocytes cultured with aminobisphosphonates and IL-2 showed a major content of IFN-γ and acquired the ability to kill tumor target cells.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2006-07-13 | International journal of immunopathology and pharmacology |