6533b823fe1ef96bd127eac3

RESEARCH PRODUCT

Protective effect of antioxidants contained in milk-based fruit beverages against sterol oxidation products

Antonio CillaGabriel López-garcíaAmparo AlegríaReyes Barberá

subject

NecrosisCell cycle checkpointCytotoxic effectCholesterol oxidation productsMedicine (miscellaneous)Milk-based fruit beverage0404 agricultural biotechnologymedicineCytotoxic T cellTX341-641Bioaccessible fractionsCaco-2 cellsOverproductionNutrition and DieteticsNutrition. Foods and food supplyChemistryPhytosterol oxidation products04 agricultural and veterinary sciences040401 food scienceIntestinal epitheliumSterolBiochemistryCaco-2medicine.symptomPlant sterolsFood Science

description

Abstract Sterol oxidation products (SOPs) have shown cytotoxic effect in human intestinal cells; however, their effect within a food matrix has not been assayed yet. This study evaluated the possible cytotoxic effect of SOPs within bioaccessible fractions (BFs) of two milk-based fruit beverages with (BFA)/without (BFB) plant sterols in differentiated Caco-2 cells and if the BFs counteracted the cytotoxic effect induced by COPs mixture (30 and 60 μM). BFs did not evoke cytotoxic effect in any of the tests carried out and they protected against the loss of intestinal cohesion, mitochondrial depolarization and necrosis induced by COPs mixture. Moreover, BFB sample protected from cell cycle arrest, lessened lysosomal injury and overproduction of ROS at 30 μM. Therefore, the formation of SOPs within milk-based fruit beverages with/without plant sterols does not contribute to intestinal epithelium damage and these types of food matrices confer additional protection against COPs at dietary concentrations.

yearjournalcountryeditionlanguage
2017-03-01Journal of Functional Foods
https://doi.org/10.1016/j.jff.2016.12.028