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RESEARCH PRODUCT
Nanoparticles and antigen-specific T-cell therapeutics: A comprehensive study on uptake and release
Volker MailänderSimone ThomasAnna JürchottMatthias TheobaldEva DistlerUmaporn PaiphansiriOliver ZupkeWolfgang HerrMartin DassUdo F. HartwigKatharina Landfestersubject
CD4-Positive T-LymphocytestumorMaterials sciencePolymersT cellBiomedical EngineeringT lymphocytesMedicine (miscellaneous)BioengineeringDevelopmentCD8-Positive T-LymphocytesEndocytosisFlow cytometryCell LineCell therapyDrug Delivery SystemsMicroscopy Electron TransmissionIn vivocell imagingmedicineNP releaseAnimalsHumansGeneral Materials ScienceDrug CarriersMicroscopy Confocalmedicine.diagnostic_testVesicletechnology industry and agricultureleukemiacellular therapyEndocytosisMice Inbred C57BLDrug Liberationmedicine.anatomical_structureImmunologyDrug deliverydrug deliveryBiophysicsnanoparticlesNanocarriersdescription
Aim: T lymphocytes are used as cellular therapeutics in many disease entities including cancer. We investigated the uptake and retention of nanoparticles (NPs) by these nonphagocytic cells. Materials & methods: Uptake, release and toxicity of various polymeric NP preparations were analyzed by flow cytometry, confocal laser scanning microscopy and transmission electron microscopy. T-cell effector functions were measured using IFN-γ-ELISPOT and 51Chromium-release assays. Results: Amino-functionalized NPs were efficiently ingested by antigen-specific T cells without adversely influencing effector functions. NPs were stored in membrane-surrounded vesicles, with major proportions released extracellularly during 24 h. Conclusion: Amino-functionalized polymeric NPs are efficiently taken up by human T cells and could be used to design nanocarriers for direct access and manipulation of antigen-specific T cells in vivo.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2015-05-02 |