6533b823fe1ef96bd127ebac

RESEARCH PRODUCT

Procedure for the Screening of Eggs and Egg Products to Detect Oxolonic Acid, Ciprofloxacin, Enrofloxacin, and Sarafloxacin Using Micellar Liquid Chromatography

Samuel Carda-brochPooja MishraDaniel García-ferrerJaume Albiol-chivaJosep Esteve-romeroAbhilasha DurgbanshiJuan Peris-vicenteDevasish Bose

subject

Microbiology (medical)Analytelaying hen01 natural sciencesBiochemistryMicrobiologyArticlechemistry.chemical_compoundSarafloxacinOxolinic acidEnrofloxacinmedicinePharmacology (medical)General Pharmacology Toxicology and PharmaceuticsTriethylamineDetection limitvalidationResidue (complex analysis)Chromatography010405 organic chemistryChemistry010401 analytical chemistrylcsh:RM1-950solid-to-liquid extraction0104 chemical sciencesfood safetyInfectious Diseaseslcsh:Therapeutics. PharmacologyMicellar liquid chromatographyfluorescenceoptimizationmedicine.drug

description

A method based on micellar liquid chromatography was developed to determine oxolinic acid, ciprofloxacin, enrofloxacin, and sarafloxacin in eggs and egg products. The antimicrobial drugs were obtained in a micellar solution which was directly injected. The analytes were resolved using a C18 column and a mobile phase of 0.05 M sodium dodecyl sulfate—7.5% 1-propanol—0.5% triethylamine, buffered at pH 3 with phosphate salt, running under the isocratic mode. The signal was monitored by fluorescence. Validation was successfully performed according to the EU Commission Decision 2002/657/EC in terms of specificity, calibration range (LOQ to 1 mg/kg), linearity (R2 &gt

yearjournalcountryeditionlanguage
2019-11-15Antibiotics
10.3390/antibiotics8040226https://www.mdpi.com/2079-6382/8/4/226