6533b823fe1ef96bd127edcb
RESEARCH PRODUCT
Improved sample preparation for the testosterone hydroxylation assay using disposable extraction columns
Franz OeschMichael ArandHelga WagnerK L Plattsubject
ChromatographyChromatography10050 Institute of Pharmacology and Toxicology610 Medicine & health1600 General ChemistryGeneral ChemistryHydroxylationHigh-performance liquid chromatographyHydroxylationchemistry.chemical_compoundchemistry570 Life sciences; biologySample preparationTestosteroneQuantitative analysis (chemistry)description
The preparation of samples for injection into a high-performance liquid chromatograph from assay mixtures for the determination of cytochrome P-450-dependent testosterone hydroxylation has been substantially facilitated. By replacing the multiple cumbersome extraction steps of the conventional method with a single column extraction the time for sample preparation was reduced from hours to minutes. The new procedure also yields better recoveries for most of the testosterone metabolites than the original protocol. The use of extraction columns for sample preparation allows the simultaneous treatment of a large number of samples or even the automation of the whole assay procedure. The modified procedure is a straightforward, easy-to-perform method that should greatly facilitate the implementation of the testosterone hydroxylation assay for sharply discriminating between many individual cytochrome P-450 species in routine enzyme diagnostics.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1992-11-06 |