6533b823fe1ef96bd127edd5

RESEARCH PRODUCT

Impact of Consuming Extra-Virgin Olive Oil or Nuts within a Mediterranean Diet on DNA Methylation in Peripheral White Blood Cells within the PREDIMED-Navarra Randomized Controlled Trial: A Role for Dietary Lipids

Miguel ÁNgel Martínez-gonzálezMiguel ÁNgel Martínez-gonzálezRamon EstruchRamon EstruchMontserrat FitóCristina RazquinCristina RazquinJordi Salas-salvadóJ. Alfredo MartínezAmelia MartiAmelia MartiAna ArpónEmilio RosEmilio RosFermín I. MilagroFermín I. MilagroDolores CorellaDolores CorellaJose I Riezu-boj

subject

Male0301 basic medicineTime FactorsMediterranean diet; DNA methylation; nuts; olive oil; blood cellsMediterranean dietADNPhysiologyComorbidity030204 cardiovascular system & hematologyDiet MediterraneanEpigenesis Genetic0302 clinical medicineRisk FactorsLeukocytesNutsCooking (Dried foods)Aged 80 and overchemistry.chemical_classificationDNA methylationNutrition and DieteticsMethylationMiddle AgedOli d'olivaTreatment OutcomeCpG siteCardiovascular DiseasesDNA methylationFemaleDiet HealthyMetilaciólcsh:Nutrition. Foods and food supplyPolyunsaturated fatty acidBlood cellslcsh:TX341-641nutsBiologyMethylationArticle03 medical and health sciencesMediterranean cookingMediterranean dietDiabetes mellitusCuina mediterràniamedicineHumansEpigeneticsOlive OilAgedMetabolismDNAProtective Factorsmedicine.diseaseCuina (Fruita seca)030104 developmental biologychemistrySpainCèl·lules sanguíniesCpG Islandsblood cellsEnergy MetabolismOlive oilFood Science

description

DNA methylation could be reversible and mouldable by environmental factors, such as dietary exposures. The objective was to analyse whether an intervention with two Mediterranean diets, one rich in extra-virgin olive oil (MedDiet + EVOO) and the other one in nuts (MedDiet + nuts), was influencing the methylation status of peripheral white blood cells (PWBCs) genes. A subset of 36 representative individuals were selected within the PREvención con DIeta MEDiterránea (PREDIMED-Navarra) trial, with three intervention groups in high cardiovascular risk volunteers: MedDiet + EVOO, MedDiet + nuts, and a low-fat control group. Methylation was assessed at baseline and at five-year follow-up. Ingenuity pathway analysis showed routes with differentially methylated CpG sites (CpGs) related to intermediate metabolism, diabetes, inflammation, and signal transduction. Two CpGs were specifically selected: cg01081346-CPT1B/CHKB-CPT1B and cg17071192-GNAS/GNASAS, being associated with intermediate metabolism. Furthermore, cg01081346 was associated with PUFAs intake, showing a role for specific fatty acids on epigenetic modulation. Specific components of MedDiet, particularly nuts and EVOO, were able to induce methylation changes in several PWBCs genes. These changes may have potential benefits in health; especially those changes in genes related to intermediate metabolism, diabetes, inflammation and signal transduction, which may contribute to explain the role of MedDiet and fat quality on health outcomes. This work was supported by CIBERobn (CB12/03/30002 to J.A.M.) and Ministerio de Economía y Competitividad (AGL2013-45554-R to J.A.M. and F.I.M.) and Red PREDIMED-RETIC RD06/0045. A.A. was supported by a grant from Centre for Nutrition Research (Universidad de Navarra) until august 2016, and from that day, by a “Formación de Profesorado Universitario” fellowship from Ministerio de Educación, Cultura y Deporte (FPU15/02790).

yearjournalcountryeditionlanguage
2017-12-23
http://hdl.handle.net/2445/156046