6533b823fe1ef96bd127ee08
RESEARCH PRODUCT
Yeasts vectored by migratory birds collected in the Mediterranean island of Ustica and description of Phaffomyces usticensis f.a. sp. nov., a new species related to the cactus ecoclade
Marco Alexandre GuerreiroGiancarlo MoschettiCláudia CarvalhoPedro AlmeidaNicola FrancescaLuca SettanniBruno MassaJosé Paulo SampaioCiro Sanninosubject
Cactaceaephenotypic characterizationMolecular Sequence DataCryptococcusAureobasidiumRhodotorulaApplied Microbiology and BiotechnologyMicrobiologyNew species Yeast birdsBirdscactus-yeastMediterranean IslandsWickerhamomycesAscomycotaGenusPhaffomyces usticensis sp. novBotanyAnimalsPhylogenyRibosomalPhylogenetic treebiologyCandida glabrataphylogenetic analysisGeneral Medicinebiology.organism_classificationnovel speciesDelichon urbicumSettore AGR/11 - Entomologia Generale E ApplicataPhenotypeRNA RibosomalCactusRNADelichon urbicum; Phaffomyces usticensis sp. nov.; cactus-yeast; novel species; phenotypic characterization; phylogenetic analysis; Animals; Ascomycota; Birds; Cactaceae; Mediterranean Islands; Molecular Sequence Data; Phenotype; Phylogeny; RNA RibosomalSettore AGR/16 - Microbiologia Agrariadescription
Nine yeast species belonging to genera Candida , Cryptococcus , Phaffomyces , Rhodotorula and Wickerhamomyces , and one species of Aureobasidium genus were isolated from the cloaca of migratory birds. Candida glabrata and C. inconspicua were the species most frequently isolated and Wickerhamomyces sylviae , which has recently been described as a new species isolated from bird cloaca, was again found. The majority of isolates showed the ability to grow up to 40 °C and/or at pH 3.0, two environmental conditions typical of the digestive tract of birds. The phylogenetic analysis of the D1/D2 domain of 26S rRNA gene placed the cultures of Phaffomyces in a new lineage that differed from the closest species, P. opuntiae , by 13 nucleotide substitutions. The new species was able to grow at 40 °C and at pH 2.5, which suggests a possible adaptation to the bird cloaca. Moreover, the ability to grow in the presence of digitonin at pH 3.7 and the assimilation of ethyl acetate indicates a potential cactophilic origin. For the first time, the presence of yeasts belonging to the Phaffomyces clade in Europe and also in non-cactus environments is reported. The new species is formally described as P. usticensis sp. nov. (PYCC 6346T = CBS 12958T).
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2014-01-01 |