6533b823fe1ef96bd127f58b

RESEARCH PRODUCT

Mechanism of Free Radical Production in Exhaustive Exercise in Humans and Rats; Role of Xanthine Oxidase and Protection by Allopurinol

Juan SastreFerrero JaCarmen DescoLance S. TeradaAndrés CuestaJohn E. RepineJose ViñaFederico V. PallardóMiguel AsensiAmparo Gimeno

subject

AdultMaleXanthine OxidaseFree RadicalsAllopurinolPhysical ExertionClinical BiochemistryAllopurinolOxidative phosphorylationallopurinolPharmacologyMitochondrionmedicine.disease_causeBiochemistrychemistry.chemical_compoundphysical exerciseMalondialdehydeGeneticsmedicineoxidative stressAnimalsHumansAspartate AminotransferasesEnzyme InhibitorsRats WistarMuscle SkeletalXanthine oxidaseCreatine KinaseExerciseMolecular BiologyOxidase testL-Lactate DehydrogenaseFree Radical ScavengersCell BiologyGlutathioneXanthineGlutathioneMitochondriaRatsOxidative StressLiverchemistryBiochemistryxanthine oxidaseOxidative stressmedicine.drug

description

Exhaustive exercise generates free radicals, However, the source of this oxidative damage remains controversial. The aim of this paper was to study further the mechanism of exercise-induced production of free radicals, Testing the hypothesis that xanthine oxidase contributes to the production of free radicals during exercise, me found not only that exercise caused an increase in blood xanthine oxidase activity in rats but also that inhibiting xanthine oxidase with allopurinol prevented exercise-induced oxidation of glutathione in both rats and in humans. Furthermore, inhibiting xanthine oxidase prevented the increases in the plasma activity of cytosolic enzymes (lactate dehydrogenase, aspartate aminotransferase, and creatine kinase) seen after exhaustive exercise. Our results provide evidence that xanthine oxidase is responsible for the free radical production and tissue damage during exhaustive exercise, These findings also suggest that mitochondria play a minor role as a source of free radicals during exhaustive physical exercise.

yearjournalcountryeditionlanguage
2000-01-01IUBMB Life (International Union of Biochemistry and Molecular Biology: Life)
https://doi.org/10.1080/15216540050167098