6533b823fe1ef96bd127f630
RESEARCH PRODUCT
Sequestration of T lymphocytes to body fluids in tuberculosis: reversal of anergy following chemotherapy.
Francesco DieliGuido SireciCaterina Di SanoAlfredo SalernoLucina TitoneRalph SpallekGiuseppe FrisciaJuraj IvanyiMahavir Singhsubject
CD4-Positive T-LymphocytesTuberculosisLipoproteinsTuberculinTuberculinEpitopeMycobacterium tuberculosisAntigenImmunology and AllergyMedicineHumansTuberculosisTuberculosis PulmonaryAntibacterial agentClonal AnergyAntigens BacterialClonal anergybiologybusiness.industryT lymphocytebiology.organism_classificationmedicine.diseaseBody FluidsInfectious DiseasesImmunologybusinessdescription
The specificity of CD4 T lymphocytes was investigated in 6 patients affected by tuberculosis who had negative tuberculin purified protein derivative (PPD) skin tests at diagnosis. Polyclonal CD4 T cell lines from the peripheral blood failed to proliferate to PPD and to the 16- or 38-kDa proteins of Mycobacterium tuberculosis, while CD4 cell lines from the disease site responded to PPD and to the 16- and 38-kDa proteins and derived epitopes in vitro. Four months after chemotherapy, the patients became responsive to PPD. The proliferative response to PPD and to the 16- or 38-kDa proteins and their derived peptides decreased in CD4 T cell lines from the disease site and increased in lines from the peripheral blood. These results indicate that CD4 T cells recognizing a vast array of M. tuberculosis epitopes are compartmentalized at the site of disease in anergic patients but appear in peripheral blood after chemotherapy.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1999-07-01 | The Journal of infectious diseases |