6533b823fe1ef96bd127f671
RESEARCH PRODUCT
A Guide to Perform 3D Histology of Biological Tissues with Fluorescence Microscopy
Annunziatina LaurinoAlessandra FranceschiniLuca PesceLorenzo CinciAlberto MontalbanoGiacomo MazzamutoGiuseppe SancataldoGabriella NesiIrene CostantiniLudovico SilvestriFrancesco Saverio Pavonesubject
3D histologyOrganic ChemistryH&General MedicineSettore FIS/07 - Fisica Applicata(Beni Culturali Ambientali Biol.e Medicin)CatalysisLight Sheet MicroscopyComputer Science ApplicationsInorganic Chemistry3D histology; clearing methods; H&E staining; Light Sheet Microscopy; Two Photon Fluorescence MicroscopyE stainingTwo Photon Fluorescence MicroscopyPhysical and Theoretical ChemistryMolecular BiologySpectroscopyclearing methodsdescription
The analysis of histological alterations in all types of tissue is of primary importance in pathology for highly accurate and robust diagnosis. Recent advances in tissue clearing and fluorescence microscopy made the study of the anatomy of biological tissue possible in three dimensions. The combination of these techniques with classical hematoxylin and eosin (H&E) staining has led to the birth of three-dimensional (3D) histology. Here, we present an overview of the state-of-the-art methods, highlighting the optimal combinations of different clearing methods and advanced fluorescence microscopy techniques for the investigation of all types of biological tissues. We employed fluorescence nuclear and eosin Y staining that enabled us to obtain hematoxylin and eosin pseudo-coloring comparable with the gold standard H&E analysis. The computational reconstructions obtained with 3D optical imaging can be analyzed by a pathologist without any specific training in volumetric microscopy, paving the way for new biomedical applications in clinical pathology.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2023-04-04 | International Journal of Molecular Sciences; Volume 24; Issue 7; Pages: 6747 |