6533b823fe1ef96bd127f67f
RESEARCH PRODUCT
Superresolved digital in-line holographic microscopy for high-resolution lensless biological imaging.
Vicente MicóZeev Zalevskysubject
Synthetic aperture radarBiomedical EngineeringHolographyHolographyOnline SystemsSensitivity and Specificitylaw.inventionBiomaterialsOpticsImaging Three-DimensionallawImage Interpretation Computer-AssistedImage resolutionLensesPhysicsMicroscopybusiness.industryPhantoms ImagingReproducibility of ResultsSignal Processing Computer-AssistedEquipment DesignHolographic interferometryImage EnhancementAtomic and Molecular Physics and OpticsElectronic Optical and Magnetic MaterialsNumerical apertureEquipment Failure AnalysisSpatial frequencybusinessBiological imagingDigital holographyAlgorithmsdescription
Digital in-line holographic microscopy (DIHM) is a modern approach capable of achieving micron-range lateral and depth resolutions in three-dimensional imaging. DIHM in combination with numerical imaging reconstruction uses an extremely simplified setup while retaining the advantages provided by holography with enhanced capabilities derived from algorithmic digital processing. We introduce superresolved DIHM incoming from time and angular multiplexing of the sample spatial frequency information and yielding in the generation of a synthetic aperture (SA). The SA expands the cutoff frequency of the imaging system, allowing submicron resolutions in both transversal and axial directions. The proposed approach can be applied when imaging essentially transparent (low-concentration dilutions) and static (slow dynamics) samples. Validation of the method for both a synthetic object (U.S. Air Force resolution test) to quantify the resolution improvement and a biological specimen (sperm cells biosample) are reported showing the generation of high synthetic numerical aperture values working without lenses.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2010-08-31 | Journal of biomedical optics |