6533b823fe1ef96bd127f7eb
RESEARCH PRODUCT
Immunocytochemical typification of mesothelial cells in effusions: in vivo and in vitro models.
M Gonzalez-devesaAntonio Ferrández-izquierdoRosario Gil-bensoAntonio Llombart-boschSamuel Navarro-fossubject
MesotheliomaPathologymedicine.medical_specialtyHistologyImmunoelectron microscopyBlotting WesternVimentinmacromolecular substancesBiologyAdenocarcinomaModels BiologicalEpitheliumPathology and Forensic MedicineMetastatic carcinomaDesminDiagnosis DifferentialImmunoenzyme TechniquesPleural diseaseCytokeratinmedicineAscitic FluidHumansVimentinCells CulturedGeneral Medicinemedicine.diseasePleural Effusionbiology.proteinAdenocarcinomaKeratinsDesminFemaleMesothelial CellFollow-Up Studiesdescription
We have performed immunocytochemical, immunoelectron microscopy, Western blot, and culture techniques using monoclonal antibodies against cytokeratin, vimentin, and desmin on 17 benign and 20 malignant effusions of pleural and ascitic origin. Triple coexpression of these three antigens was observed in benign reactive mesothelial cells as well as in one case of mesothelioma. All metastatic adenocarcinoma cells were consistently negative to desmin and positive to cytokeratin and vimentin. Present results were helpful to distinguish reactive and malignant mesothelioma from metastatic carcinoma cells in effusions.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1994-05-01 | Diagnostic cytopathology |