6533b824fe1ef96bd127ffac
RESEARCH PRODUCT
The Candida albicans cell wall-associated glyceraldehyde-3-phosphate dehydrogenase activity increases in response to starvation and temperature upshift.
M. L. DelgadoDaniel GozalboM L Gilsubject
biologymedicine.diagnostic_testImmunoelectron microscopyTemperatureGlyceraldehyde-3-Phosphate DehydrogenasesDehydrogenaseGeneral Medicinebiology.organism_classificationYeastCorpus albicansFlow cytometryCell wallFungal ProteinsInfectious DiseasesBiochemistryCell WallCandida albicansbiology.proteinmedicineCandida albicansGlyceraldehyde 3-phosphate dehydrogenasedescription
We have determined the effect of environmental factors (mild thermal upshift and starvation) on the Candida albicans cell wall-associated glyceraldehyde-3-phosphate dehydrogenase (cwGAPDH) activity. Temperature upshift (from 28 to 37 degrees C) and/ or starvation (at 28 or 37 degrees C in water) of exponentially growing yeast cells caused an increase in cwGAPDH activity (3 to 5-, and 7 to 8-fold, respectively). This increase in activity did not correlate with an increase in the amount of cwGAPDH protein present, as determined by flow cytometry, immunoelectron microscopy and Western-blotting. These results indicate that thermal upshift and starvation cause an activation of the cwGAPDH in C. albicans cells.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2002-06-11 | Medical mycology |