Multi-approach metabolomics analysis and artificial simplified phytocomplexes reveal cultivar-dependent synergy between polyphenols and ascorbic acid in fruits of the sweet cherry (Prunus avium L.).

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RESEARCH PRODUCT

Multi-approach metabolomics analysis and artificial simplified phytocomplexes reveal cultivar-dependent synergy between polyphenols and ascorbic acid in fruits of the sweet cherry (Prunus avium L.).

Stefano Negri Michael Assfalg Martino Bianconi Flavia Guzzo Gianni Zoccatelli Stefania Ceoldo Linda Avesani Francesca Munari Mauro Commisso Matteo Stocchero Stefania Poletti Alessandra Bulgarini Flavia Di Carlo

subject

0301 basic medicine antioxidant Antioxidant medicine.medical_treatment Organic chemistry lcsh:Medicine Ascorbic Acid Biochemistry 01 natural sciences Antioxidants Mass Spectrometry Analytical Chemistry Prunus Spectrum Analysis Techniques artificial phytocomplex Metabolites Vitamin C Prunus avium L.Cultivar lcsh:Science Cherries Chromatography High Pressure Liquid Liquid Chromatography Microscopy Multidisciplinary Chromatographic Techniques Light Microscopy food and beverages Vitamins Plants Physical sciences Chemistry Horticulture Italy Metabolome secondary Research Article Prunus avium L. antioxidant secondary metabolism synergy artificial phytocomplex metabolism synergy Fluorescence Recovery after Photobleaching Liquid Chromatography-Mass Spectrometry Prunus avium Biology Research and Analysis Methods Fruits Chemical compounds 03 medical and health sciences Metabolomics Species Specificity Organic compounds Botany medicine Metabolomics Genetic variability Nuclear Magnetic Resonance Biomolecular 030109 nutrition & dietetics Vitamin C 010401 analytical chemistry lcsh:R Organisms Biology and Life Sciences Polyphenols Ascorbic acid 0104 chemical sciences Metabolism Polyphenol Fruit Multiprotein Complexes Linear Models lcsh:Q

description

Fruits of the sweet cherry (Prunus avium L.) accumulate a range of antioxidants that can help to prevent cardiovascular disease, inflammation and cancer. We tested the in vitro antioxidant activity of 18 sweet cherry cultivars collected from 12 farms in the protected geographical indication region of Marostica (Vicenza, Italy) during two growing seasons. Multiple targeted and untargeted metabolomics approaches (NMR, LC-MS, HPLC-DAD, HPLC-UV) as well as artificial simplified phytocomplexes representing the cultivars Sandra Tardiva, Sandra and Grace Star were then used to determine whether the total antioxidant activity reflected the additive effects of each compound or resulted from synergistic interactions. We found that the composition of each cultivar depended more on genetic variability than environmental factors. Furthermore, phenolic compounds were the principal source of antioxidant activity and experiments with artificial simplified phytocomplexes indicated strong synergy between the anthocyanins and quercetins/ascorbic acid specifically in the cultivar Sandra Tardiva. Our data therefore indicate that the total antioxidant activity of sweet cherry fruits may originate from cultivar-dependent interactions among different classes of metabolite.

year	journal	country	edition	language
2017-07-01	PLoS ONE

10.1371/journal.pone.0180889 http://europepmc.org/articles/PMC5521804?pdf=render