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RESEARCH PRODUCT

The Influence of Hyaluronic Acid Biofunctionalization of a Bovine Bone Substitute on Osteoblast Activity In Vitro

Peer W. KämmererAndreas PabstSolomiya KyyakDiana Heimes

subject

Technology02 engineering and technologyArticleAndrology03 medical and health scienceschemistry.chemical_compound0302 clinical medicineHyaluronic acidhyaluronic acidmedicineGeneral Materials ScienceMTT assayViability assayxenograftoral regenerationBone regenerationMicroscopyQC120-168.85TbovineQH201-278.5biofunctionalizationosteoblastsOsteoblastCell migration030206 dentistrybone substituteEngineering (General). Civil engineering (General)021001 nanoscience & nanotechnologyIn vitroTK1-9971Bovine bonemedicine.anatomical_structureDescriptive and experimental mechanicschemistryElectrical engineering. Electronics. Nuclear engineeringTA1-20400210 nano-technology

description

Bovine bone substitute materials (BSMs) are used for oral bone regeneration. The objective was to analyze the influence of BSM biofunctionalization via hyaluronic acid (HA) on human osteoblasts (HOBs). BSMs with ± HA were incubated with HOBs including HOBs alone as a negative control. On days 3, 7 and 10, cell viability, migration and proliferation were analyzed by fluorescence staining, scratch wound assay and MTT assay. On days 3, 7 and 10, an increased cell viability was demonstrated for BSM+ compared with BSM− and the control (each p ≤ 0.05). The cell migration was enhanced for BSM+ compared with BSM− and the control after day 3 and day 7 (each p ≤ 0.05). At day 10, an accelerated wound closure was found for the control compared with BSM+/− (each p &lt

yearjournalcountryeditionlanguage
2021-05-27Materials
10.3390/ma14112885http://dx.doi.org/10.3390/ma14112885