0000000000384251

AUTHOR

Solomiya Kyyak

showing 4 related works from this author

Possible Implications for Improved Osteogenesis? The Combination of Platelet-Rich Fibrin With Different Bone Substitute Materials

2021

Bone substitute materials (BSM) are widely used in oral regeneration, but sufficient angiogenesis is crucial for osteogenesis. The combination of BSM with autologous thrombocyte concentrations such as platelet-rich fibrin (PRF) may represent a clinical approach to overcome this limitation. This study analyzes the early influence on osteoblast (HOB) in vitro. Here, four different BSM (allogeneic, alloplastic, and two of xenogeneic origin) were combined with PRF. After the incubation with osteoblasts for 24 h, cell viability, migration, and proliferation were assessed. Next, marker of proliferation, migration, and differentiation were evaluated on gene and protein levels in comparison to the …

allograftHistologylcsh:BiotechnologyBiomedical Engineeringplatelet-rich fibrinBioengineering02 engineering and technologyBone morphogenetic proteinBone morphogenetic protein 2Andrology03 medical and health sciences0302 clinical medicineTissue engineeringlcsh:TP248.13-248.65medicineViability assayxenograftoral regenerationOriginal ResearchChemistryBioengineering and BiotechnologyOsteoblast030206 dentistrybone substitute021001 nanoscience & nanotechnologyPlatelet-rich fibrinRUNX2medicine.anatomical_structuretissue engineeringosteoblastAlkaline phosphatase0210 nano-technologyBiotechnologyFrontiers in Bioengineering and Biotechnology
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The Influence of Hyaluronic Acid Biofunctionalization of a Bovine Bone Substitute on Osteoblast Activity In Vitro

2021

Bovine bone substitute materials (BSMs) are used for oral bone regeneration. The objective was to analyze the influence of BSM biofunctionalization via hyaluronic acid (HA) on human osteoblasts (HOBs). BSMs with ± HA were incubated with HOBs including HOBs alone as a negative control. On days 3, 7 and 10, cell viability, migration and proliferation were analyzed by fluorescence staining, scratch wound assay and MTT assay. On days 3, 7 and 10, an increased cell viability was demonstrated for BSM+ compared with BSM− and the control (each p ≤ 0.05). The cell migration was enhanced for BSM+ compared with BSM− and the control after day 3 and day 7 (each p ≤ 0.05). At day 10, an accelerated wound…

Technology02 engineering and technologyArticleAndrology03 medical and health scienceschemistry.chemical_compound0302 clinical medicineHyaluronic acidhyaluronic acidmedicineGeneral Materials ScienceMTT assayViability assayxenograftoral regenerationBone regenerationMicroscopyQC120-168.85TbovineQH201-278.5biofunctionalizationosteoblastsOsteoblastCell migration030206 dentistrybone substituteEngineering (General). Civil engineering (General)021001 nanoscience & nanotechnologyIn vitroTK1-9971Bovine bonemedicine.anatomical_structureDescriptive and experimental mechanicschemistryElectrical engineering. Electronics. Nuclear engineeringTA1-20400210 nano-technologyMaterials
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Activation of Human Osteoblasts via Different Bovine Bone Substitute Materials With and Without Injectable Platelet Rich Fibrin in vitro

2021

IntroductionThe aim of the in vitro study was to compare the effect of four bovine bone substitute materials (XBSM) with and without injectable platelet-reach fibrin for viability and metabolic activity of human osteoblasts (HOB) as well as expression of alkaline phosphatase (ALP), bone morphogenetic protein 2 (BMP-2), and osteonectin (OCN).Materials and MethodsCerabone® (CB), Bio-Oss® (BO), Creos Xenogain® (CX) and MinerOss® X (MO) ± i-PRF were incubated with HOB. At day 3, 7, and 10, cell viability and metabolic activity as well as expression of ALP, OCN, and BMP-2, was examined.ResultsFor non-i-PRF groups, the highest values concerning viability were seen for CB at all time points. Pre-t…

Histologyplatelet rich fibrin (PRF)proliferationlcsh:BiotechnologyBiomedical EngineeringBioengineering02 engineering and technologyBone morphogenetic protein 2vitalityFibrinAndrology03 medical and health sciences0302 clinical medicinelcsh:TP248.13-248.65medicineViability assaybovine boneOriginal ResearchbiologyChemistryBioengineering and Biotechnologyin vitroOsteoblastbone substitute030206 dentistry021001 nanoscience & nanotechnologydigestive system diseasesPlatelet-rich fibrinIn vitroPCRmedicine.anatomical_structureosteoblastbiology.proteinAlkaline phosphataseOsteonectin0210 nano-technologyBiotechnologyFrontiers in Bioengineering and Biotechnology
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Combination of an allogenic and a xenogenic bone substitute material with injectable platelet-rich fibrin – A comparative in vitro study

2020

The aim of the in vitro study was a comparison of an allogenic (ABSM) and a xenogenic bone substitute material (XBSM) with and without injectable platelet-rich fibrin (ABSM-i-PRF & XBSM-i-PRF) on cell characteristics of human osteoblasts (HOB). Here, ABSM and XBSM (+ i-PRF = test; - i-PRF = control) were incubated with HOB for 3, 7 and 10 days. HOB viability, migration, proliferation and differentiation (RT-PCR on alkaline phosphatase (AP), bone morphogenetic protein 2 (BMP-2) and osteonectin (OCN)) were measured and compared between groups. At day 3, an increased viability, migration and proliferation was seen for ABSM-i-PRF. For viability and proliferation (days 7 and 10) and for mig…

Bone substituteBiomedical EngineeringPharmacologyFibrinCell LineInjectionsBiomaterials03 medical and health sciences0302 clinical medicineCell MovementPlatelet-Rich FibrinHumansIn vitro studyCell Proliferation030304 developmental biology0303 health sciencesOsteoblastsbiologyChemistryCell Differentiation030206 dentistrydigestive system diseasesPlatelet-rich fibrinIn vitroBone Substitutesbiology.proteinJournal of Biomaterials Applications
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