Activation of Human Osteoblasts via Different Bovine Bone Substitute Materials With and Without Injectable Platelet Rich Fibrin in vitro

6533b83afe1ef96bd12a7173

RESEARCH PRODUCT

Activation of Human Osteoblasts via Different Bovine Bone Substitute Materials With and Without Injectable Platelet Rich Fibrin in vitro

Solomiya Kyyak Sebastian Blatt Eik Schiegnitz Diana Heimes Henning Staedt Henning Staedt Daniel G. E. Thiem Keyvan Sagheb Bilal Al-nawas Peer W. Kämmerer

subject

Histology platelet rich fibrin (PRF)proliferation lcsh:Biotechnology Biomedical Engineering Bioengineering 02 engineering and technology Bone morphogenetic protein 2 vitality Fibrin Andrology 03 medical and health sciences 0302 clinical medicine lcsh:TP248.13-248.65 medicine Viability assay bovine bone Original Research biology Chemistry Bioengineering and Biotechnology in vitro Osteoblast bone substitute 030206 dentistry 021001 nanoscience & nanotechnology digestive system diseases Platelet-rich fibrin In vitro PCR medicine.anatomical_structure osteoblast biology.protein Alkaline phosphatase Osteonectin 0210 nano-technology Biotechnology

description

IntroductionThe aim of the in vitro study was to compare the effect of four bovine bone substitute materials (XBSM) with and without injectable platelet-reach fibrin for viability and metabolic activity of human osteoblasts (HOB) as well as expression of alkaline phosphatase (ALP), bone morphogenetic protein 2 (BMP-2), and osteonectin (OCN).Materials and MethodsCerabone® (CB), Bio-Oss® (BO), Creos Xenogain® (CX) and MinerOss® X (MO) ± i-PRF were incubated with HOB. At day 3, 7, and 10, cell viability and metabolic activity as well as expression of ALP, OCN, and BMP-2, was examined.ResultsFor non-i-PRF groups, the highest values concerning viability were seen for CB at all time points. Pre-treatment with i-PRF increased viability in all groups with the highest values for CB-i-PRF after 3 and 7 and for CX-i-PRF after 10 days. For metabolic activity, the highest rate among non-i-PRF groups was seen for MO at day 3 and for CB at day 7 and 10. Here, i-PRF groups showed higher values than non-i-PRF groups (highest values: CB + i-PRF) at all time points. There was no difference in ALP-expression between groups. For OCN expression in non-i-PRF groups, CB showed the highest values after day 3, CX after day 7 and 10. Among i-PRF-groups, the highest values were seen for CX + i-PRF. At day 3, the highest BMP-2 expression was observed for CX. Here, for i-PRF groups, the highest increase was seen for CX + i-PRF at day 3. At day 7 and 10, there was no significant difference among groups.ConclusionXBSM sintered under high temperature showed increased HOB viability and metabolic activity through the whole period when compared to XBSM manufactured at lower temperatures. Overall, the combination of XBSM with i-PRF improved all cellular parameters, ALP and BMP-2 expression at earlier stages as well as OCN expression at later stages.

year	journal	country	edition	language
2021-02-01	Frontiers in Bioengineering and Biotechnology

https://doi.org/10.3389/fbioe.2021.599224