6533b824fe1ef96bd1280171
RESEARCH PRODUCT
Glucosylation of isatin-3-oxime followed by 2D in situ NMR in plant cells at highest magnetic field without labelling.
Christiane HinseMatthias UngerJoachim StöckigtAlessandro Provenzanisubject
In situIsatinGlycosylationAnalytical chemistryCatalysisMass SpectrometryRauwolfiaGlucosidesRauvolfia serpentinaLabellingCulture TechniquesNuclear Magnetic Resonance BiomolecularChromatography High Pressure LiquidChromatographyPlants MedicinalbiologyMolecular StructureChemistrybeta-GlucosidaseMetabolismNuclear magnetic resonance spectroscopyPlant cellbiology.organism_classificationCarbonMagnetic fieldMolecular MedicineIsatin-3-oximeHydrogendescription
The glucosylation of isatin-3-oxime (1) was monitored by in situ 2D 1H-13C inverse correlated gradient assisted NMR spectroscopy in plant cell suspension cultures of Rauvolfia serpentina without labelling. The applied high magnetic field of 800 MHz allowed measurements within 20 min at concentrations of 1 of 5.76 mM. Complete glucosylation of 1 occurs inside the cells within 72 hours. During this time isatin-3-oxime-glucoside (2) accumulates without further metabolism.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2001-04-01 | Natural product letters |