6533b824fe1ef96bd12809b7

RESEARCH PRODUCT

Single hollow fiber SLM extraction of polyamines followed by tosyl chloride derivatization and HPLC determination

Piotr WieczorekJan ÅKe JönssonKatarzyna Dziarkowska

subject

hollow fiber membraneTime FactorspolyaminesBiochemistryHigh-performance liquid chromatographyAnalytical ChemistryTosyl Compoundschemistry.chemical_compoundEnvironmental ChemistryHumansSample preparationFibersupported liquid membraneDerivatizationSpectroscopyChromatography High Pressure LiquidChromatographyextraction techniqueExtraction (chemistry)Reproducibility of ResultsMembranes ArtificialHydrogen-Ion ConcentrationAcceptor2 × 2 real matriceschemistryHollow fiber membrane

description

Determination of polyamines in biological fluids possesses medical diagnostic relevance. Despite the vast panel of analytical methods developed for polyamines they are not applied in routine clinical usage, mainly due to the time and labor consuming sample preparation step and complicated derivatization procedures. Thus, new simpler methods are needed. This paper describes a single hollow fiber SLM extraction method of polyamines followed by simple pre-column derivatization with tosyl chloride and HPLC-UV analysis. The influence of different parameters such as the extraction time, organic phase composition, acceptor pH, donor pH, acceptor volume, donor volume and stirring speed on the transport parameters and enrichment was studied and discussed. The optimized method was applied to real matrices such as urine and plasma.

yearjournalcountryeditionlanguage
2007-10-11Analytica Chimica Acta
10.1016/j.aca.2007.11.014https://www.sciencedirect.com/science/article/pii/S0003267007018697#!