6533b824fe1ef96bd1280be8
RESEARCH PRODUCT
Enzyme-responsive intracellular-controlled release using silica mesoporous nanoparticles capped with ε-poly-L-lysine.
Félix SancenónAlessandro AgostiniRamón Martínez-máñezEnrique Pérez-payáPedro AmorósMar OrzáezCristina De La TorreNúria MasLaura MondragónVicente Pelechano Ferragudsubject
Silicon dioxideNanoparticlemesoporous materialsCatalysisRutheniumchemistry.chemical_compoundHydrolysisQUIMICA ORGANICACell Line TumorQUIMICA ANALITICAOrganic chemistryHumansPolylysineColoring Agentschemistry.chemical_classificationintracellular releaseOrganic ChemistryQUIMICA INORGANICAGeneral ChemistryPolymerMesoporous silicaSilicon DioxideControlled releaseCombinatorial chemistrychemistryPolylysineDelayed-Action Preparationsanchoring strategyNanoparticlesnanoparticlesMesoporous materialLysosomesPorositypoly-L-lysineHeLa Cellsdescription
The synthesis and characterization of two new capped silica mesoporous nanoparticles for controlled delivery purposes are described. Capped hybrid systems consist of MCM-41 nanoparticles functionalized on the outer surface with polymer epsilon-poly-L-lysine by two different anchoring strategies. In both cases, nanoparticles were loaded with model dye molecule [Ru(bipy)(3)](2+). An anchoring strategy involved the random formation of urea bonds by the treatment of propyl isocyanate-functionalized MCM-41 nanoparticles with the lysine amino groups located on the epsilon-poly-L-lysine backbone (solid Ru-rLys-S1). The second strategy involved a specific attachment through the carboxyl terminus of the polypeptide with azidopropyl-functionalized MCM-41 nanoparticles (solid Ru-tLys-S1). Once synthesized, both nanoparticles showed a nearly zero cargo release in water due to the coverage of the nanoparticle surface by polymer epsilon-poly-L-lysine. In contrast, a remarkable payload delivery was observed in the presence of proteases due to the hydrolysis of the polymer's amide bonds. Once chemically characterized, studies of the viability and the lysosomal enzyme-controlled release of the dye in intracellular media were carried out. Finally, the possibility of using these materials as drug-delivery systems was tested by preparing the corresponding epsilon-poly-L-lysine capped mesoporous silica nanoparticles loaded with cytotoxic drug camptothecin (CPT), CPT-rLys-S1 and CPT-tLys-S1. Cellular uptake and cell-death induction were studied. The efficiency of both nanoparticles as new potential platforms for cancer treatment was demonstrated.
year | journal | country | edition | language |
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2014-04-25 | Chemistry (Weinheim an der Bergstrasse, Germany) |