Search results for "poly-L-lysine"

showing 4 items of 4 documents

Enhanced Efficacy and Broadening of Antibacterial Action of Drugs via the Use of Capped Mesoporous Nanoparticles

2013

[EN] A novel nanodevice consisting of mesoporous nanoparticles loaded with vancomycin and capped with epsilon-poly-L-lysine (epsilon-PL) was prepared and its interaction with different Gram-negative bacteria studied. A remarkable improvement in the efficacy of the antimicrobial drug epsilon-PL and a broadening of the antimicrobial spectrum of vancomycin is demonstrated.

INGENIERIA DE LA CONSTRUCCIONNanoparticleNanotechnologyMicrobial Sensitivity TestsCatalysisQUIMICA ORGANICAVancomycinGram-Negative BacteriaQUIMICA ANALITICABIOQUIMICA Y BIOLOGIA MOLECULARmedicinePolylysineGated materialsNanodeviceDrug CarriersChemistryQUIMICA INORGANICAOrganic ChemistryGeneral ChemistrySilicon DioxideAntimicrobialMesoporous materialsAnti-Bacterial AgentsAntimicrobial drugepsilon-poly-L-lysineNanoparticlesVancomycinAntibacterial actionMesoporous materialPorositymedicine.drugChemistry - A European Journal
researchProduct

Stable polyplexes based on arginine-containing oligopeptides for in vivo gene delivery.

2004

In this study, we investigated to what extent the stability and transduction capacity of polyplexed DNA can be improved by optimizing the condensing peptide sequence. We have synthesized a small library of cationic peptides, at which the lysine/arginine ratio and the cation charge were varied. All peptides were able to compact DNA, at which polyplexes of short lysine-rich sequences were considerably larger than those of elongated or arginine-rich peptides (GM102 and GM202). In addition, the arginine-rich peptides GM102 and GM202 rendered the polyplexes resistant to plasma incubation or DNase I-mediated digestion. While all peptides were found to improve the transfection efficiency in HepG2 …

MaleChemical PhenomenaLysineGenetic VectorsMolecular Sequence DataPeptideGene deliveryBiologyArginineTransfectionTransduction (genetics)MiceDrug StabilityTransduction GeneticGeneticsAnimalsDeoxyribonuclease IHumansTissue DistributionAmino Acid SequenceMolecular BiologyPeptide sequencechemistry.chemical_classificationSettore MED/04 - Patologia GeneraleOligopeptideChemistry PhysicalGene Transfer TechniquesTransfectionPeptide FragmentsMice Inbred C57BLcondensationBiochemistrychemistrypolyplexDNase I protectionGene TargetingMolecular MedicineDeoxyribonuclease IpolyethyleneimineOligopeptidespoly-L-lysine
researchProduct

Nanocomplexes for gene therapy of respiratory diseases: Targeting and overcoming the mucus barrier

2015

Gene therapy, i.e. the delivery and expression of therapeutic genes, holds great promise for congenital and acquired respiratory diseases. Non-viral vectors are less toxic and immunogenic than viral vectors, although they are characterized by lower efficiency. However, they have to overcome many barriers, including inflammatory and immune mediators and cells. The respiratory and airway epithelial cells, the main target of these vectors, are coated with a layer of mucus, which hampers the effective reaching of gene therapy vectors carrying either plasmid DNA or small interfering RNA. This barrier is thicker in many lung diseases, such as cystic fibrosis. This review summarizes the most impor…

Pulmonary and Respiratory MedicineCystic FibrosisGenetic enhancementContext (language use)Gene deliveryVectors in gene therapyPolyethylene GlycolsViral vectorPolyethyleinimine Poly-L-lysine Ethylene glycol Chitosan PAMAM G0 dendrimer N-(1-(23-Dioleyloxy)propyl)-NNNtrimethylammonium chloride 12-Dioleoylphosphatidylethanolamine N-acetylcystein 12-Dioctadecanoyl-sn-glycero-3-phosphoethanolaminemedicineHumansTechnology PharmaceuticalPharmacology (medical)RNA Small InterferingLungExpectorantsInflammationLungbusiness.industryBiochemistry (medical)Gene Transfer TechniquesGenetic TherapyMucusMucusmedicine.anatomical_structureSettore CHIM/09 - Farmaceutico Tecnologico ApplicativoImmunologyNanoparticlesInflammation MediatorsbusinessPlasmidsRespiratory tract
researchProduct

Enzyme-responsive intracellular-controlled release using silica mesoporous nanoparticles capped with ε-poly-L-lysine.

2014

The synthesis and characterization of two new capped silica mesoporous nanoparticles for controlled delivery purposes are described. Capped hybrid systems consist of MCM-41 nanoparticles functionalized on the outer surface with polymer epsilon-poly-L-lysine by two different anchoring strategies. In both cases, nanoparticles were loaded with model dye molecule [Ru(bipy)(3)](2+). An anchoring strategy involved the random formation of urea bonds by the treatment of propyl isocyanate-functionalized MCM-41 nanoparticles with the lysine amino groups located on the epsilon-poly-L-lysine backbone (solid Ru-rLys-S1). The second strategy involved a specific attachment through the carboxyl terminus of…

Silicon dioxideNanoparticlemesoporous materialsCatalysisRutheniumchemistry.chemical_compoundHydrolysisQUIMICA ORGANICACell Line TumorQUIMICA ANALITICAOrganic chemistryHumansPolylysineColoring Agentschemistry.chemical_classificationintracellular releaseOrganic ChemistryQUIMICA INORGANICAGeneral ChemistryPolymerMesoporous silicaSilicon DioxideControlled releaseCombinatorial chemistrychemistryPolylysineDelayed-Action Preparationsanchoring strategyNanoparticlesnanoparticlesMesoporous materialLysosomesPorositypoly-L-lysineHeLa CellsChemistry (Weinheim an der Bergstrasse, Germany)
researchProduct