6533b824fe1ef96bd1280cfb
RESEARCH PRODUCT
The biomaterial polyphosphate blocks stoichiometric binding of the SARS-CoV-2 S-protein to the cellular ACE2 receptor
Heinz C. SchröderMeik NeufurthXiaohong WangWerner E. G. MüllerHadrian ScheplerEmad TolbaShunfeng Wangsubject
ArgininePolymersBiomedical EngineeringAntiviral Agents03 medical and health scienceschemistry.chemical_compound0302 clinical medicinePolyphosphatesotorhinolaryngologic diseasesmedicineHumansGeneral Materials ScienceReceptor030304 developmental biologychemistry.chemical_classification0303 health sciencesInnate immune systemBinding SitesChemistryPolyphosphateBiomaterialChemical modificationHeparinPolyelectrolytesdigestive system diseases3. Good healthAmino acidMolecular Docking SimulationBiochemistry030220 oncology & carcinogenesisSpike Glycoprotein CoronavirusAngiotensin-Converting Enzyme 2medicine.drugProtein Bindingdescription
The effect of the polyanionic polymer of inorganic polyphosphate (polyP) involved in innate immunity on the binding of the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein to the cellular ACE2 receptor was studied. The RBD surface comprises a basic amino acid stretch of four arginine residues which interact with the physiological polyP (polyP40) and polyP3. Subsequently, the interaction of RBD with ACE2 is sensitively inhibited. After the chemical modification of arginine, an increased inhibition by polyP, at a 1 : 1 molar ratio (polyP : RBP), is measured already at 0.1 μg mL−1. Heparin was ineffective. The results suggest a potential therapeutic benefit of polyP against SARS-CoV-2 infection.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2020-11-25 | Biomaterials Science |