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RESEARCH PRODUCT

Influence of pro- and prebiotics on gastric, duodenal and colonic bioaccessibility of the mycotoxin beauvericin

subject

description

Abstract Beauvericin (BEA) is a bioactive compound produced by the secondary metabolism of several Fusarium strains and known to have various biological activities. This study investigates the influence of several dietary fibers (galactomanan, glucomannan, citrus fiber, bamboo fiber, carrot fiber, pie fiber, β-glucan, xilan, and cellulose) and probiotic strains ( Lactobacillus animalis , Lb. casei , Lb. casei , Lb. plantarum , Lb. rhuminis , Lb. casei casei , Bifidobacterium breve , Bf. Adolescents , Bf. bifidum , Corynebacterium vitaeruminis , Streptococcus faecalis , Eubacterium crispatus , and Saccharomyces cerevisiae ) on the minor Fusarium mycotoxin BEA bioaccessibility employing a model solution. The bioaccessibility was determined using a simulated gastrointestinal digestion that mimics the physiological conditions of the digestive tract until the colonic compartment. The determination of BEA in the intestinal fluids was carried out by liquid chromatography–mass spectrometry detection (LC–MS). The reduction of BEA bioaccessibility in the experiments carried out using the prebiotic compounds ranged from 60 to 80%, whereas in the trials carried out using the probiotic strains the bioaccessibility observed ranged from 30 to 85%. A BEA degradation product produced by colonic fermentation was identified using the technique of LC–MS-LIT.