6533b824fe1ef96bd1281629

RESEARCH PRODUCT

Calcium transport in rat small intestine in vitro and in vivo

subject

description

Intestinal calcium (Ca) transport was studied in the rat jejunum by the in vitro perfusion technique of Fisher and Parsons and in the tied loop in vivo. Mucosal uptake and absorption of Ca was examined under the following conditions: rising intraluminal Ca-concentrations (0.5–128 meq/l); inhibition of energy dependent metabolism (2,4-dinitrophenol, N2, low temperature); net water flow, out of or into the intestinal lumen; addition of strontium (Sr); pretreatment with low Ca-diet and with 6-methyl-prednisolone. The concentration-dependent Ca absorption curve rose steeply at low Ca-concentrations but changed to a slowly rising straight line above 16 meq/l Ca++. In contrast, Ca uptake into the intestinal wall was directly related to Ca concentration, was linear from the beginning and paralleled the straight part of the absorption curve. Ca absorption was decreased by inhibition of energy dependent metabolism, addition of Sr and pretreatment with prednisolone. Pretreatment with low Ca diet increased Ca absorption and direction of net water flow (“solvent drag”) had no effect on it. Mucosal uptake of Ca was similar to Ca absorption except that metabolic inhibition increased Ca uptake but decreased Ca absorption. These results are compatible with the concept of a passive mucosal uptake and of an active absorption of Ca at low intraluminal Ca concentrations with additional passive component at high Ca concentrations.