6533b825fe1ef96bd1281e81

RESEARCH PRODUCT

Determination of multiple mycotoxins in feedstuffs by combined use of UPLC–MS/MS and UPLC–QTOF–MS

Eva M. MateoJosé V. GómezDavid RomeraR. Mateo-castroJosé Vicente Gimeno-adelantadoMisericordia Jiménez

subject

Ochratoxin AAflatoxinMass spectrometryFumonisins01 natural sciencesMass SpectrometryDiacetoxyscirpenolAnalytical Chemistrychemistry.chemical_compound0404 agricultural biotechnologyAflatoxinsMycotoxinZearalenoneChromatography High Pressure LiquidChromatography010401 analytical chemistry04 agricultural and veterinary sciencesGeneral MedicineMycotoxinsAnimal FeedOchratoxins040401 food science0104 chemical sciencesT-2 ToxinchemistryUplc qtof msZearalenoneUplc ms msTrichothecenesFood Science

description

In this report, a UPLC-ESI-MS/MS method for the simultaneous determination of aflatoxins, ochratoxin A, zearalenone, deoxynivalenol, fumonisins, T-2 and HT-2 toxins, fusarenone X, diacetoxyscirpenol, and 3- and 15-acetyldeoxynivalenol in feedstuffs was developed. A quadrupole-time-of-flight mass spectrometer detector (QTOF-MS) operating in full scan mode was combined with the UPLC-ESI-MS/MS system to confirm the identity of detected mycotoxins and to identify other possible microbial metabolites occurring in samples. Sixty-two feed samples from the Spanish market were analyzed. Extraction of metabolites was carried out with acetonitrile-water-formic acid (80:19:1, v/v/v). Method detection and quantification limits and performance criteria set by Commission Regulation (EC) No 401/2006 were fulfilled. Relatively high levels of the main regulated mycotoxins and presence of non-regulated mycotoxins in feed samples were found. This is the first study in which mycotoxins and other microbial metabolites occurring in feed are studied using a UPLC-QTOF-MS system being therefore a reference report.

yearjournalcountryeditionlanguage
2018-11-01Food Chemistry
https://doi.org/10.1016/j.foodchem.2017.11.040