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RESEARCH PRODUCT
First report of Tomato leaf curl Sinaloa virus infecting tomato crops in Panama
J. A. Herrera-vásquezLuis C. MejíaD. OrtegaSalvatore DavinoStefano PannoMario DavinoA.b. Romerosubject
PanamaSolanum lycopersicum viral plant diseasebiologyHealth Toxicology and Mutagenesisfungifood and beveragesSettore AGR/12 - Patologia VegetalePlant Sciencebiology.organism_classificationHorticultureAgronomyPlant virusTomato leaf curl Sinaloa virusSolanumAgronomy and Crop ScienceMixed infectiondescription
In April 2011 and September 2012, virus-like symptoms were observed in open field- and greenhouse-grown tomato crops (Solanum lycopersicum) in Chiriquí, the westernmost province of Panama. Samples from symptom-bearing plants (127 in all) were collected and tested for the presence of begomoviruses by polymerase chain reaction (PCR) assays with sets of degenerated primers designed to amplify parts of the DNA-A and DNA-B components (Rojas et al., 1993; Table 1). Products of the expected sizes, obtained with both DNA-A- and DNA-B-specific primers for 49 samples, suggested infection with New World bipartite begomoviruses. This corresponds to an incidence of 26% (8 plants) in open field, and 43% (41 plants) in greenhouse crops. Primers specific for ten tomato-infecting begomoviruses found in Central America (Engel et al., 1998; Nakhla et al., 2005; Table 1) were used to typify the PCR-positive samples.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2015-01-01 |