0000000000401101
AUTHOR
J. A. Herrera-vásquez
Begomoviruses Infecting Tomato Crops in Panama
The key regions in Panama involved in open field- and greenhouse-grown commercial tomato production, including the Chiriquí, Veraguas, Herrera, Los Santos, Coclé and Panama Oeste provinces, were surveyed for the incidence and distribution of begomoviruses in the growing seasons of 2011 and 2012. The surveys took place in 14 of the 51 districts of the above-mentioned provinces and comprised all relevant tomato production areas of the provinces. A total of 28 tomato plots were surveyed. The exact location of each plot was geo-referenced using a hand-held Global Positioning System unit. In total, 319 individual tomato plants (181 in 2011 and 138 in 2012) were sampled. Plants displayed diverse …
First report of Tomato leaf curl Sinaloa virus infecting tomato crops in Panama
In April 2011 and September 2012, virus-like symptoms were observed in open field- and greenhouse-grown tomato crops (Solanum lycopersicum) in Chiriquí, the westernmost province of Panama. Samples from symptom-bearing plants (127 in all) were collected and tested for the presence of begomoviruses by polymerase chain reaction (PCR) assays with sets of degenerated primers designed to amplify parts of the DNA-A and DNA-B components (Rojas et al., 1993; Table 1). Products of the expected sizes, obtained with both DNA-A- and DNA-B-specific primers for 49 samples, suggested infection with New World bipartite begomoviruses. This corresponds to an incidence of 26% (8 plants) in open field, and 43% …
Genetic diversity ofMelon necrotic spot virusandOlpidiumisolates from different origins
The geographic incidence, genetic diversity and phylogenetic relationships of Melon necrotic spot virus (MNSV) and Olpidium isolates were studied in three cucurbit species from several Latin American and European countries on different collecting dates. Of the 112 cucurbit samples analysed, 69 from Guatemala, Honduras, Mexico, Panama and Spain were DAS-ELISA-positive for MNSV. Olpidium bornovanus and O. virulentus infections, and MNSV infections mixed with these Olpidium species, were observed for all these countries. Twenty-nine MNSV isolates from all the origins where the virus was detected were selected and amplified by RT-PCR. The resulting RT-PCR of the p29, p89, p7A, p7B and p42 prote…