6533b825fe1ef96bd1281f72

RESEARCH PRODUCT

Rapid whole protein quantitation of staphylococcal enterotoxins A and B by liquid chromatography/mass spectrometry

Jordi MañesJosé Miguel SorianoCarla SolerIsabel Sospedra

subject

Spectrometry Mass Electrospray IonizationComplete proteinEnterotoxinmedicine.disease_causeBiochemistryAnalytical ChemistryEnterotoxinsLimit of DetectionLiquid chromatography–mass spectrometrymedicineAnimalsPathogenDetection limitChromatography Reverse-PhaseChromatographyChemistryOrganic ChemistryReproducibility of ResultsGeneral MedicineReversed-phase chromatographyStaphylococcal Food PoisoningMilkStaphylococcus aureusFruitLinear ModelsCitrus sinensis

description

Abstract Staphylococcus aureus is an important pathogen and has been indicated as the fifth causative agent of food-borne human illness throughout the world. Staphylococcal enterotoxins (SEs) are toxic compounds excreted mainly by strains of S. aureus. Among these toxins, enterotoxins A (SEA) and B (SEB) are both of the most prevalent compounds in staphylococcal food poisoning. In this work, reverse phase liquid chromatography coupled to ESI mass spectrometry (LC–ESI/MS) has been applied for its rapid identification and quantification. Limit of detection (LOD) values were 0.5 and 0.2 ng for SEA and SEB, respectively and limit of quantification (LOQ) value was 1 ng for both enterotoxins. SEA and SEB have been analyzed as intact proteins in milk and fruit juices. Analytical methods are essential for routine monitoring purposes and safeguard public health and the proposed technique can detect and quantify successfully SEA and SEB in food samples.

yearjournalcountryeditionlanguage
2012-05-01Journal of Chromatography A
https://doi.org/10.1016/j.chroma.2012.03.022