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RESEARCH PRODUCT
The POT1 gene for yeast peroxisomal thiolase is subject to three different mechanisms of regulation
José E. Pérez-ortínLuis FrancoJ. Carlos IgualCarmen González-boschsubject
Transcriptional ActivationTranscription GeneticGenes FungalSaccharomyces cerevisiaeMutantOleic AcidsSaccharomyces cerevisiaeMicrobodiesMicrobiologyGene Expression Regulation FungalGene expressionRNA MessengerAcetyl-CoA C-AcetyltransferaseMolecular BiologyGeneRegulation of gene expressionbiologyCell CycleFungal geneticsRNA FungalPeroxisomebiology.organism_classificationChromatinChromatinGlucoseBiochemistryOleic Aciddescription
The Saccharomyces cerevisiae POT1 gene is, as are other yeast peroxisomal protein genes, inducible by fatty acids and repressible by glucose. We have now found that it is also induced during the stationary phase of the culture. To investigate these three regulatory circuits, we have studied the mRNA levels of regulatory mutants as well as the changes in chromatin structure upon gene activation. We conclude that the regulation of transcriptional activity in glucose repression, oleate induction, and stationary phase induction follow different molecular mechanisms. We suggest that this multiplicity of regulatory mechanisms may represent a general rule for the yeast peroxisomal protein genes.
year | journal | country | edition | language |
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1992-07-01 | Molecular Microbiology |