6533b825fe1ef96bd1282029
RESEARCH PRODUCT
Gene expression is circular: factors for mRNA degradation also foster mRNA synthesis.
Mordechai ChoderOren BarkaiSebastián ChávezManuel GarberSebastien CausseGal HaimovichJosé E. Pérez-ortínDaniel A. MedinaXavier DarzacqGonzalo Millán-zambranosubject
CytoplasmSaccharomyces cerevisiae ProteinsTranscription GeneticRNA StabilityGenes FungalRNA polymerase IIRNA-binding proteinSaccharomyces cerevisiaeGenètica molecularGeneral Biochemistry Genetics and Molecular Biology03 medical and health sciencesGene Expression ProcessTranscription (biology)Gene Expression Regulation FungalGene expressionP-bodiesmedicineRNA Messenger030304 developmental biologyRegulation of gene expressionCell Nucleus0303 health sciencesbiologyBiochemistry Genetics and Molecular Biology(all)030302 biochemistry & molecular biologyRNA-Binding ProteinsRNA FungalMolecular biologyCell biologyCell nucleusmedicine.anatomical_structureExoribonucleasesbiology.proteinRNARNA Polymerase IIdescription
SummaryMaintaining proper mRNA levels is a key aspect in the regulation of gene expression. The balance between mRNA synthesis and decay determines these levels. We demonstrate that most yeast mRNAs are degraded by the cytoplasmic 5′-to-3′ pathway (the “decaysome”), as proposed previously. Unexpectedly, the level of these mRNAs is highly robust to perturbations in this major pathway because defects in various decaysome components lead to transcription downregulation. Moreover, these components shuttle between the cytoplasm and the nucleus, in a manner dependent on proper mRNA degradation. In the nucleus, they associate with chromatin—preferentially ∼30 bp upstream of transcription start-sites—and directly stimulate transcription initiation and elongation. The nuclear role of the decaysome in transcription is linked to its cytoplasmic role in mRNA decay; linkage, in turn, seems to depend on proper shuttling of its components. The gene expression process is therefore circular, whereby the hitherto first and last stages are interconnected.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2013-05-01 | Cell |