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RESEARCH PRODUCT

Flashing light emitting diodes (LEDs) induce proteins, polyunsaturated fatty acids and pigments in three microalgae

Ralf RautenbergerTamára SantosLisa M. SchülerDaniela Morales-sánchezPeter S.c. SchulzePeter S.c. SchulzeHugo PereiraRené H. WijffelsRené H. WijffelsViswanath KironSerena LimaFrancesca ScargialiJoão Varela

subject

0106 biological sciences0301 basic medicinePigmentsLuteinBio Process EngineeringTotal lipidsSettore ING-IND/25 - Impianti ChimiciBioengineering01 natural sciencesApplied Microbiology and Biotechnology03 medical and health scienceschemistry.chemical_compoundPigment:Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Plantefysiologi: 492 [VDP]NeoxanthinPulsed lightChlorophytaVDP::Teknologi: 500::Bioteknologi: 590010608 biotechnologyVDP::Technology: 500::Biotechnology: 590MicroalgaeFood scienceBiomassCarotenoidVLAGchemistry.chemical_classificationDuty cycleDuty cycle Pigments PUFA Pulsed light Total lipidsFatty Acidsfood and beveragesGeneral Medicine:Matematikk og Naturvitenskap: 400::Basale biofag: 470::Molekylærbiologi: 473 [VDP]Flashing030104 developmental biologychemistryChlorophyllvisual_artvisual_art.visual_art_mediumFatty Acids Unsaturated:Teknologi: 500::Bioteknologi: 590 [VDP]StramenopilesPUFABiotechnologyPolyunsaturated fatty acidViolaxanthin

description

As the periodic emission of light pulses by light emitting diodes (LEDs) is known to stimulate growth or induce high value biocompounds in microalgae, this flashing light regime was tested on growth and biochemical composition of the microalgae Nannochloropsis gaditana, Koliella antarctica and Tetraselmis chui. At low flashing light frequencies (e.g., 5 and 50 Hz, Duty cycle = 0.05), a strain-dependent growth inhibition and an accumulation of protein, polyunsaturated fatty acids, chlorophyll or carotenoids (lutein, β-carotene, violaxanthin and neoxanthin) was observed. In addition, a 4-day application of low-frequency flashing light to concentrated cultures increased productivities of eicosapentaenoic acid (EPA) and specific carotenoids up to three-fold compared to continuous or high frequency flashing light (500 Hz, Duty cycle = 0.05). Therefore, applying low-frequency flashing light as finishing step in industrial production can increase protein, polyunsaturated fatty acids or pigment contents in biomass, leading to high-value algal products.

yearjournalcountryeditionlanguage
2020-11-25
10.1016/j.jbiotec.2020.11.019https://hdl.handle.net/10037/21257